Nucleotide sequences of eight human natural autoantibody VH regions reveals apparent restricted use of VH families.
Open Access
- 1 June 1989
- journal article
- research article
- Published by The American Association of Immunologists in The Journal of Immunology
- Vol. 142 (11), 4054-4061
- https://doi.org/10.4049/jimmunol.142.11.4054
Abstract
Eight full length cDNA were isolated from EBV transformed human PBL derived from different normal individuals. Five were derived from antibodies with the characteristics of natural polyreactive antibodies. Three were either monoreactive or bireactive. The most striking feature of the structure of these molecules was their utilization of VH families. Although three used the large VHIII family and one used the large VHI family, the other four used genes derived from two of the recently defined small human VH families VHIV and VHV. Three of the molecules represent VHIV expressed sequences and one is the first example of a VHV gene used in an antibody of defined specificity. The nucleotide sequences of some of the molecules were remarkably similar in their VH gene segments to previously described VH genes. The data suggest that natural autoantibodies may use a restricted portion of the VH repertoire, and, in addition, that some polyreactive antibodies may be germ line encoded. The implication of these findings for the origin and diversity of the human B cell repertoire is discussed.This publication has 22 references indexed in Scilit:
- A novel family of variable region genes of the human immunoglobulin heavy chainJournal of Molecular Biology, 1987
- Human Lymphocytes Making Rheumatoid Factor and Antibody to ssDNA Belong to Leu-1 + B-Cell SubsetScience, 1987
- Human Monoclonals from Antigen-Specific Selection of B Lymphocytes and Transformation by EBVScience, 1986
- Organization and evolution of variable region genes of the human immunoglobulin heavy chainJournal of Molecular Biology, 1986
- A simple and very efficient method for generating cDNA librariesGene, 1983
- Production of autoantibodies by human-human hybridomas.JCI Insight, 1982
- Organization, structure, and assembly of immunoglobulin heavy chain diversity DNA segments.The Journal of Experimental Medicine, 1982
- Human immunoglobulin D segments encoded in tandem multigenic familiesNature, 1981
- Structure of the human immunoglobulin μ locus: Characterization of embryonic and rearranged J and D genesCell, 1981
- Structure and multiplicity of genes for the human immunoglobulin heavy chain variable region.Proceedings of the National Academy of Sciences, 1980