Hypoosmotic Dialysis and Ultrafiltration Technique for Preparation of Mammalian Hemoglobin: a Comparison of Three Species
- 1 January 1988
- journal article
- research article
- Published by Taylor & Francis in Biomaterials, Artificial Cells and Artificial Organs
- Vol. 16 (5), 887-904
- https://doi.org/10.3109/10731198809117275
Abstract
Dialysis of human, bovine, and ovine red blood cells using a hypotonic solution and a commercial kidney dialysis unit, followed by ultrafiltration through 0.1 micron pore hollow-fibers provides an easily managed method for isolation of lipid-free hemoglobin (LFHB). High performance liquid chromatography (HPLC) analysis of hemoglobin indicated 99% protein purity. SDS polyacrylamide gel electrophoresis analysis demonstrated that LFHB migrates as a single band. Processing one-half liter of packed red blood cells requires approximately 10 hours and resulting in an average of 93% hemoglobin recovery.Keywords
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