A comparison of two modifications of Rappaport's enrichment medium (R25 and RV) for the isolation of salmonellas from sewage polluted natural water
- 1 December 1983
- journal article
- research article
- Published by Cambridge University Press (CUP) in Epidemiology and Infection
- Vol. 91 (3), 451-458
- https://doi.org/10.1017/s0022172400060496
Abstract
The development of Rappaport''s enrichment medium and elevated temperature incubation as methods of salmonella isolation is traced. The recent recording of a merger of the 2 techniques by means of Rappaport-Vassiliadis medium is noted (RV medium). In Cardiff [Wales, UK], an earlier modification of Rappaport''s enrichment (R25) by Vassiliadis was found to be efficient in salmonella recovery from environment samples. The 2 media were compared using sewage polluted natural water as test material. Under the conditions of experiment, R25 was more successful in salmonella isolation than RV, although the latter medium inhibited competitive organisms better. R25 is a convenient enrichment broth for routine use. In combination with pre-enrichment it allows the use of a loop for subculture rather than a pipette. This increases safe manipulation. It also produces a high proportion of positive isolations at the 24 h subculture time in contrast to other enrichment broths. For these reasons it forms an integral part of salmonella isolation methodology.This publication has 36 references indexed in Scilit:
- Dynamics of salmonella isolation with modified Rappaport's medium (R10)Journal of Applied Bacteriology, 1983
- The Rappaport—Vassiliadis (RV) enrichment medium for the isolation of salmonellas: An overviewJournal of Applied Bacteriology, 1983
- A comparison of the R25 modification of Rappaport's enrichment medium with strontium chloride B for salmonella isolation from sewage polluted natural waterEpidemiology and Infection, 1982
- Comparison of selenite F, Muller-Kauffmann tetrathionate and Rappaport's medium for the isolation of salmonellas from sewage-polluted natural water using a pre-enrichment techniqueEpidemiology and Infection, 1979
- Principles of Salmonella IsolationJournal of Applied Bacteriology, 1979
- Salmonella surveillance with reference to pigs – Cardiff abattoir, 1968–1975Epidemiology and Infection, 1977
- An improved method of isolating salmonellae from contaminated desiccated coconutJournal of Clinical Pathology, 1964
- THE ISOLATION OF SALMONELLAEJournal of Applied Bacteriology, 1962
- A TRIAL OF RAPPAPORT'S MEDIUMJournal of Clinical Pathology, 1958
- Laboratory findings in enteric group fevers in the Middle East forcesEpidemiology and Infection, 1946