PORTAL VENOUS TRANSFUSION UP-REGULATES KUPFFER CELL CYCLOOXYGENASE ACTIVITY
- 1 July 1997
- journal article
- Published by Wolters Kluwer Health in Transplantation
- Vol. 64 (1), 135-139
- https://doi.org/10.1097/00007890-199707150-00023
Abstract
Portal venous transfusions (PVTs) of blood have been shown to induce significant immunosuppression in animal models of organ transplantation. A proposed mechanism of PVT-induced immunosuppression is via alteration of Kupffer cell arachidonic acid metabolism with increased secretion of the suppressive metabolite prostaglandin E2 (PGE2). This study assessed the hypothesis that PVT increases Kupffer cell PGE2 production via up-regulation of Kupffer cell phospholipase A2 (PLA2) as well as constitutive (COX1) and inducible (COX2) cyclooxygenase. Kupffer cells from Lewis rats were harvested 1 hr after PVT with either 1 ml of Wistar-Furth blood, systemic transfusion (SVT), or saline via portal vein (PVSal). After lipopolysaccharide stimulation, 24-hr Kupffer cell supernatant fractions were assayed for PGE2. PGE2 was increased after SVT (1465±234 pg/ml) compared with PVSal (597±99; PKeywords
This publication has 21 references indexed in Scilit:
- PROLONGATION OF RAT SMALL BOWEL OR RENAL ALLOGRAFT SURVIVAL BY PRETRANSPLANT TRANSFUSION AND/OR BY VARYING THE ROUTE OF ALLOGRAFT VENOUS DRAINAGETransplantation, 1994
- ARGININE-SPECIFIC SUPPRESSION OF MIXED LYMPHOCYTE CULTURE REACTIVITY BY KUPFFER CELLS—A BASIS OF PORTAL VENOUS TOLERANCETransplantation, 1991
- ABROGATION OF THE INDUCTION OF PORTAL VENOUS TOLERANCE IN A CARDIAC TRANSPLANT MODEL RESULTING FROM KUPFFER CELL INHIBITION BY GADOLINIUMTransplantation, 1990
- THE EFFECTS OF PERIOPERATIVE PORTAL VENOUS INOCULATION WITH DONOR LYMPHOCYTES ON RENAL ALLOGRAFT SURVIVAL IN THE RATTransplantation, 1990
- IMMUNOREGULATTON OF TRANSFUSION-INDUCED IMMUNOSUPPRESSION WITH INHIBITORS OF THE ARACHIDONIC ACID METABOLISMTransplantation, 1989
- KUPFFER CELL BLOCKADE INHIBITS INDUCTION OF TOLERANCE BY THE PORTAL VENOUS ROUTETransplantation, 1989
- EVIDENCE THAT DONOR SPLEEN CELLS ADMINISTERED THROUGH THE PORTAL VEIN PROLONG THE SURVIVAL OF CARDIAC ALLOGRAFTS IN RATSTransplantation, 1988
- TOLERANCE INDUCTION OF ALLOREACTIVITY BY PORTAL VENOUS INOCULATION WITH ALLOGENEIC CELLS FOLLOWED BY THE INJECTION OF CYCLOPHOSPHAMIDETransplantation, 1987
- IMPORTANCE OF PREOPERATIVE (NOT PEROPERATIVE) TRANSFUSIONS FOR CADAVER KIDNEY TRANSPLANTSTransplantation, 1981
- Number and evolutionary conservation of α- and β-tubulin and cytoplasmic β- and γ-actin genes using specific cloned cDNA probesCell, 1980