Subnanosecond fluorescence quenching of dye molecules in solution

Abstract

Subnanosecond fluorescence kinetics of malachite green and erythrosin were measured. The fluorescence lifetime of malachite green depends on the viscosity of the solution as η2/3. The fluorescence lifetime of erythrosin decreases linearly as the concentration of water increases in a water–acetone mixture. The fluorescence quenching of malachite green and erythrosin is attributed to environmental effects on the nonradiative internal conversion and intersystem crossing rates, respectively.