Weitere Untersuchungen über den Abbau der Aminosäuren im Tierkörper.

Abstract

Rabbit''s, guinea pig''s and dog''s kidney proved more satisfactory than that of the rat for de-aminizing amino acids. Oxidation of the ketonic acids produced from glutamic acid and aspartic acid occurs so readily as to prevent their isolation. Such destruction was effectively checked by poisoning the kidney tissue with m/1000 arsenious acid. Under such conditions glutamic acid produced [alpha]-keto-glutaric acid which was isolated as 2,4-dinitrophenylhydrazone (m. p. 222[degree] (un-corrected)). Aspartic acid was converted chiefly into pyruvic acid, and to a lesser degree also into oxalacetic acid; the former was isolated as the 2,4-dinitrophenylhydrazone, the latter proven present by its reaction with aniline. Formation of ammonia was increased when the kidney tissue was poisoned by arsenious or hydrocyanic acid.