Production in Mice of Large Volumes of Ascites Fluid Containing

Abstract

Mice injected intraperitoneally with either egg albumin or bovine serum albumin mixed in Freund''s adjuvant develop large amounts of peritoneal fluid containing antibodies to the antigen injected. As much aa 28 ml of fluid was collected from a single mouse on a single tapping. Most mice, however, produce from 0.5-14 ml of fluid each. About 50% of the mice injected respond with accumulation of fluid in the peritoneum. A pool of fluids containing 213 [mu] of antibody N/ml was used to produce passive anaphylaxis in mice, and as little as 10 [mu] of antibody N is sufficient to sensitize pertussis-treated mice.