Identification of Some OH Radical-induced Products of Lysozyme
- 1 January 1983
- journal article
- research article
- Published by Taylor & Francis in International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine
- Vol. 43 (2), 185-193
- https://doi.org/10.1080/09553008314550211
Abstract
OH radical reactions with lysozyme in gamma-irradiated N2O saturated aqueous solutions caused formation of allo-threonine, alpha-amino-n-butyric acid, o- and m- tyrosines, and 2- and 3-hydroxytyrosines. These identified radiolytic products were characterized by capillary gas chromatography-mass spectrometry as their trimethylsilyl derivatives after HCl-hydrolysis of irradiated lysozyme. Their initial G-values were also determined using gas chromatography. The possible use of these radiolytic products as monitors of radiation-induced damage to proteins and the sites of attack are also discussed.Keywords
This publication has 14 references indexed in Scilit:
- Kováts' indices of trimethylsilylated amino acids on fused-silica capillary columnsJournal of Chromatography A, 1982
- The radioracemization of amino acids by ionizing radiation: Geochemical and cosmochemical implicationsOrigins of Life, 1979
- Radiation chemistry of amino acids and peptides in aqueous solutionsJournal of Agricultural and Food Chemistry, 1978
- The Three-Dimensional Structure of an Enzyme MoleculeScientific American, 1966
- On the Radiolysis of Methionine in Aqueous Solution by Gamma IrradiationJournal of Radiation Research, 1966
- Pulse Radiolysis Studies. I. Transient Spectra and Reaction-Rate Constants in Irradiated Aqueous Solutions of BenzeneThe Journal of Chemical Physics, 1962
- THE RADIOLYSIS OF AQUEOUS CALCIUM BENZOATE AND BENZOIC ACID SOLUTIONS1The Journal of Physical Chemistry, 1960
- Degradation of DL-Methionine by RadiationNature, 1958
- Oxidation of Aromatic Compounds in Aqueous Solution by Free Radicals Produced by Photo-excited Electron Transfer in Iron Complexes1Journal of the American Chemical Society, 1953
- 89. Chemical actions of ionising radiations on aqueous solutions. Part VIII. Hydroxylation of benzoic acid by free radicals produced by X-raysJournal of the Chemical Society, 1951