The soil food web of two beech forests ( Fagus sylvatica ) of contrasting humus type: stable isotope analysis of a macro- and a mesofauna-dominated community

Abstract

The structure of the soil food web in two beech (Fagus sylvatica) forests, the Göttinger Wald and the Solling forest (Northern Germany), was investigated using variations in tissue ¹⁵N concentrations of animal species or taxa. The Göttinger Wald is located on a limestone plateau and characterized by mull humus with high macrofauna activity, particularly of Lumbricidae, Diplopoda and Isopoda. In contrast, the Solling forest is located on a sandstone mountain range and characterized by moder humus. The soil fauna of this forest is dominated by mesofauna, particularly by Collembola, Enchytraeidae and Oribatida. In June 1995 soil fauna was sampled using heat extraction. Three soil layers were analysed at each of the sites. ¹⁵N/¹⁴N ratios of bulk material increased strongly with soil depth in both forests. This also applied to the water-soluble fraction at the Göttinger Wald, but not at the Solling. Generally, the water-soluble fraction was more enriched in ¹⁵N than the bulk materials. For most animals studied ¹⁵N/¹⁴N ratios varied little with soil depth. In both forests soil animals could be classified either as saprophages, including microphytophages, or predators. On average, the δ¹⁵N of predatory taxa (Chilopoda, Araneida, Gamasina, Staphylinidae) exceeded that of saprophagous or microphytophagous taxa (Lumbricidae, Isopoda, Diplopoda, Collembola, Oribatida, Enchytraeidae) by 4.4 and 3.9‰ for the Göttinger Wald and the Solling, respectively. We assume that most of the saprophagous or microphytophagous taxa studied consist of primary and secondary decomposers and hypothesize that predators prey more on secondary than primary decomposers. Generally, average δ¹⁵N values differed little between saprophagous (Lumbricidae, Diplopoda, Isopoda) and microphytophagous taxa (Collembola, Oribatida). The variations in δ¹⁵N values of species within these taxa consistently exceeded the variation between them, indicating that the species of each of these taxa form a continuum from primary to secondary decomposers. Also, variations in δ¹⁵N values within predatory taxa in most cases exceeded that between taxa excluding top predators like Sorex. We conclude that using higher taxonomic units in soil food web analysis is problematic and in general not consistent with nature. Higher taxonomic units may only be useful for depicting very general trophic groupings such as predators or microbi-detritivores.