Abstract
The incorporation of 32P-orthophosphate into the phospholipids of H. diminuta was followed for up to 4 h in vitro. The rates and patterns of incorporation of 32P into phosphatidc acid, phosphoinositide, phosphatidylserine, lecithin, lysolecithin, and phosphatidylethanolamine plus cardiolipin indicated that H. diminuta possesses mechanisms for the de novo synthesis of these phospholipids. The major pathway utilized by H. diminuta in fatty acid incorporation was via phosphatidic acid.