Solution structure of a zinc domain conserved in yeast copper-regulated transcription factors
- 1 July 1998
- journal article
- Published by Springer Science and Business Media LLC in Nature Structural & Molecular Biology
- Vol. 5 (7), 551-555
- https://doi.org/10.1038/805
Abstract
The three dimensional structure of the N-terminal domain (residues 1-42) of the copper-responsive transcription factor Amtl from Candida glabrata has been determined by two-dimensional 1H-correlated nuclear magnetic resonance (NMR) methods. The domain contains an array of zinc-binding residues (Cys-X2-Cys-X8-Cys-X-His) that is conserved among a family of Cu-responsive transcription factors. The structure is unlike those of previously characterized zinc finger motifs, and consists of a three-stranded antiparallel beta-sheet with two short helical segments that project from one end of the beta-sheet. Conserved residues at positions 16, 18 and 19 form a basic patch that may be important for DNA binding.Keywords
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