Human Gi protein α‐subunit: deduction of amino acid structure from a cloned cDNA
- 26 January 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 211 (2), 160-164
- https://doi.org/10.1016/0014-5793(87)81428-x
Abstract
The amino acid sequence of the α‐subunit of Gi, the human adenylate cyclase inhibiting GTP‐binding protein, has been deduced from the nucleotide sequence of a DNA clone complementary to Giα, mRNA from differentiated U937 cells. The cDNA encodes a polypeptide of 355 amino acids (M r 40456). The amino acid sequence homology between human Giα and rat, murine, and bovine Giα is 98.6, 97.7 and 87.9% respectively. Differentiation of the U937 cells from monoblasts to monocyte‐like cells resulted in a 3‐fold increase in Giα mRNA as well as a 3.6‐fold increase in the 41 kDa pertussis toxin substrate presumed to be Giα. Thus, increased levels of this G‐protein are associated with monocyte differentiation and appear to be regulated transcriptionally.Keywords
This publication has 23 references indexed in Scilit:
- β‐Subunits of the human liver Gs/Gi signal‐transducing proteins and those of bovine retinal rod cell transducin are identicalFEBS Letters, 1986
- Primary structure of the α‐subunit of bovine adenylate cyclase‐inhibiting G‐protein deduced from the cDNA sequenceFEBS Letters, 1986
- Primary structure of the α‐subunit of bovine adenylate cyclase‐stimulating G‐protein deduced from the cDNA sequenceFEBS Letters, 1986
- Primary structure of the α-subunit of transducin and its relationship to ras proteinsNature, 1985
- Homologies Between Signal Transducing G Proteins and ras Gene ProductsScience, 1984
- G proteins and dual control of adenylate cyclaseCell, 1984
- Isolation of biologically active ribonucleic acid from sources enriched in ribonucleaseBiochemistry, 1979
- Empirical Predictions of Protein ConformationAnnual Review of Biochemistry, 1978
- 3′ Non-coding region sequences in eukaryotic messenger RNANature, 1976
- Detection of specific sequences among DNA fragments separated by gel electrophoresisJournal of Molecular Biology, 1975