Calvasculin, as a factor affecting the microfilament assemblies in rat fibroblasts transfected by src gene
- 7 June 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 324 (1), 51-55
- https://doi.org/10.1016/0014-5793(93)81530-d
Abstract
Cell transformations accompany alterations in cell morphology and microfilament patterns. Calvasculin encodes mRNA termed pEL-98, 18A2, 42A, p9Ka, or mtsl, found to be elevated in several metastatic cell lines. We report the elevation of calvasculin expression in SR-3Y1 cells, which show disappearance of ordered microfilaments, compared to that in 3Y1 cells and that the similar distribution of calvasculin to that of actin filaments. Interestingly, calvasculin co-sediments with F-actin and bundles actin filaments in a Ca2+-dependent manner. This activity, along with the elevation of calvasculin following transformation, suggests that the disorganization of filaments in SR-3Y1 cell is due to the cross-linking activity of calvasculin.Keywords
This publication has 18 references indexed in Scilit:
- Isolation and characterization of a calcium-binding protein derived from mRNA termed p9Ka, pEL-98, 18A2, or 42A by the newly synthesized vasorelaxant W-66 affinity chromatographyArchives of Biochemistry and Biophysics, 1992
- Isolation and characterization of a gene specifically expressed in different metastatic cells and whose deduced gene product has a high degree of homology to a Ca2+-binding protein family.Genes & Development, 1989
- Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDaAnalytical Biochemistry, 1987
- Intracellular localization of the 55-kD actin-bundling protein in cultured cells: spatial relationships with actin, alpha-actinin, tropomyosin, and fimbrin.The Journal of cell biology, 1986
- Actin-binding proteins—regulators of cell architecture and motilityNature, 1982
- Actin-binding proteinsTrends in Biochemical Sciences, 1982
- S100 protein is present in cultured human malignant melanomasNature, 1980
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Actin, alpha-actinin, and tropomyosin interaction in the structural organization of actin filaments in nonmuscle cells.The Journal of cell biology, 1976
- The effects of reciprocal changes in temperature on the transformed state of cells infected with a Rous sarcoma virus mutantVirology, 1971