Furocoumarins from Angelica dahurica with Hepatoprotective Activity on Tacrine-Induced Cytotoxicity in Hep G2 Cells

Abstract

Fractionation of the MeOH extract of Angelica dahurica Benth et Hook resulted in the isolation of six furocoumarins, imperatorin (1), isoimperatorin (2), (±)-byakangelicol (3), (+)-oxypeucedanin (4), (+)-byakangelicin (5), and (+)-aviprin (6). Among these, compounds 1 and 5 exhibited strong hepatoprotective activities, displaying EC₅₀ values of 36.6 ± 0.98 and 47.9 ± 4.6 μM, respectively. Compounds 3 and 4 showed moderate activities with EC₅₀ values of 112.7 ± 5.35 and 286.7 ± 6.36 μM, respectively. Silybin as a positive control showed the EC₅₀ value with 69.0 ± 3.4 μM. Comparison of hepatoprotective activities for six furocoumarins 1 - 6 suggested that oxy-substitution at the C-9 position increased the hepatoprotective activity.