Die Substratspezifität ,,anorganischer“ Poly- und Metaphosphatasen. II. Trennung der Enzyme

Abstract

Yeast maceration juice con-tains enzymes hydrolysing pyrophosphate, tri- and tetrapoly-phosphate, Graham''s salt, and tri- and tetrametaphosphate. Pyrophosphatase and tripolyphosphatase were separated from the other enzymes by precipitation at pH 4.1; these two enzymes were separated by paper electrophoresis. The enzyme hydrolyz-ing tetrapolyphosphate, tetrametaphosphate and Graham'' s salt were inactivated by "autoproteolysis"; they were distinguished by their relative adsorptions on alumina and on calcium bento-nite. The metaphosphatases were precipitated by between 20-40% saturated ammonium sulfate, while the polyphosphatases were precipitated by between 40 and 60%.