Radioiodination of Antibodies Adsorbed to Insoluble Antigens
- 1 January 1967
- journal article
- research article
- Published by The American Association of Immunologists in The Journal of Immunology
- Vol. 98 (1), 67-71
- https://doi.org/10.4049/jimmunol.98.1.67
Abstract
A method for radiolodinating antibody adsorbed to an insoluble antigen was developed. Antifibrin antibody as measured by its adsorption to fibrin was found to be sensitive to iodination. Antifibrin adsorption activity was preserved if iodination was carried out while the antibody was adsorbed to fibrin. Subsequent elution and readsorption showed little loss in activity. A fibrinophilic factor from normal globulin with a relatively high adsorption capacity was insensitive to iodination and was therefore unlike antifibrin antibody. A naturally occurring fibrinophilic protein, readily iodinated and elutable, was disclosed by the technique for labeling insoluble components.This publication has 8 references indexed in Scilit:
- The in Vitro Absorption of Anti-Brain Radioantibodies with Brain Sediments: Some Unusual Properties and a Quantitative MethodThe Journal of Immunology, 1967
- Variations in Affinities of Antibodies during the Immune Response*Biochemistry, 1964
- Vascular Relationships of Tumor and Host*Published by S. Karger AG ,1964
- The Effect of Iodination on Antibody ActivityThe Journal of Immunology, 1960
- Localization of Radioiodinated Rat Fibrinogen in Transplanted Rat Tumors2JNCI Journal of the National Cancer Institute, 1959
- A GENERAL METHOD FOR THE LABELING OF THE ACTIVE SITE OF ANTIBODIES AND ENZYMESProceedings of the National Academy of Sciences, 1959
- LOCALIZATION INVIVO OF RADIOIODINATED ANTI-RAT-FIBRIN ANTIBODIES AND RADIOIODINATED RAT FIBRINOGEN IN THE MURPHY RAT LYMPHOSARCOMA AND IN OTHER TRANSPLANTABLE RAT TUMORS1959
- The Immunologic Reactivity of Bovine Serum Albumin Labelled with Trace-Amounts of Radioactive Iodine(I131)The Journal of Immunology, 1949