Nucleic Acid-Functionalized Pt Nanoparticles: Catalytic Labels for the Amplified Electrochemical Detection of Biomolecules
Top Cited Papers
- 18 February 2006
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 78 (7), 2268-2271
- https://doi.org/10.1021/ac0519864
Abstract
Nucleic acid-functionalized Pt nanoparticles (Pt-NPs) act as catalytic labels for the amplified electrochemical detection of DNA hybridization and aptamer/protein recognition. Hybridization of the nucleic acid-modified Pt-NPs with a sensing nucleic acid/analyte DNA complex associated with an electrode enables the amperometric, amplified, detection of the DNA by the Pt NP electrocatalyzed reduction of H2O2 (sensitivity limit, 1 × 10-11 M). Similarly, the association of aptamer-functionalized Pt- NPs to a thrombin aptamer/thrombin complex associated with an electrode allowed the amplified, electrocatalytic detection of thrombin with a sensitivity limit corresponding to 1 × 10-9 M.Keywords
This publication has 12 references indexed in Scilit:
- Investigation of Molecular Beacon Aptamer‐Based Bioassay for Platelet‐Derived Growth Factor DetectionChemBioChem, 2005
- Aptamer-Functionalized Au Nanoparticles for the Amplified Optical Detection of ThrombinJournal of the American Chemical Society, 2004
- Electrochemical DNA sensorsNature Biotechnology, 2003
- Electrochemical DNA Hybridization BiosensorsElectroanalysis, 2002
- Efficient Near-Infrared Polymer Nanocrystal Light-Emitting DiodesScience, 2002
- Redox-Active Nucleic-Acid Replica for the Amplified Bioelectrocatalytic Detection of Viral DNAJournal of the American Chemical Society, 2002
- Amplified detection of DNA and analysis of single-base mismatches by the catalyzed deposition of gold on Au-nanoparticlesThe Analyst, 2001
- Enzyme-Linked Amplified Electrochemical Sensing of Oligonucleotide−DNA Interactions by Means of the Precipitation of an Insoluble Product and Using Impedance SpectroscopyLangmuir, 1999
- In vitro selection of RNA molecules that bind specific ligandsNature, 1990
- The development of an immunosensor for the electrochemical determination of the isoenzyme LDH5Biosensors, 1989