GLYCOSAMINOGLYCAN DISTRIBUTION IN RABBIT AORTIC-WALL FOLLOWING BALLOON CATHETER DEENDOTHELIALIZATION - AN ULTRASTRUCTURAL-STUDY
- 1 January 1980
- journal article
- research article
- Vol. 43 (6), 509-516
Abstract
The glycosaminoglycan (GAG) content of rabbit aortic wall was assayed morphometrically using ruthenium red-stained sections viewed by transmission electron microscopy. Two types of ruthenium red-positive granules were identified in the intercellular matrix. Large granules (20-50 nm in diameter) with interconnecting fibrils were removed by digestion of the tissue with testicular hyaluronidase or chondroitinase ABC. Smaller granules (20 nm) were localized mainly in endothelial basement membrane and were resistant to digestion by these enzymes. Both types of granules were removed from the tissue by the action of nitrous acid. At intervals of 11-17 wk after a single balloon deendothelialization of the aortas of normolipemic rabbits, there were significantly more granules of both types in areas where endothelium regenerated over the neointima than in areas not covered by endothelium. Lipid accumulated preferentially in the endothelium-covered neointima. Compared to normal aortas, there is an increase in the large granule content in the reendothelialized areas and a decrease in large and small granule content in the nonendothelialized areas. GAG may trap low density lipoproteins. The low GAG content of the nonendothelialized neointima may account for the low lipid content; the lack of endothelial cover may allow diffusion of GAG out of these areas carrying low density lipoprotein into the blood stream in the form of GAG-low density lipoprotein complexes or low density lipoprotein removal may be facilitated by high density lipoprotein.This publication has 21 references indexed in Scilit:
- Effect of regenerated endothelium on lipid accumulation in the arterial wall.Proceedings of the National Academy of Sciences, 1977
- The ground substance of the arterial wall Part 2. Electron-microscopic studiesAtherosclerosis, 1976
- ELECTRON-MICROSCOPIC FEATURES OF LIPOPROTEIN-GLYCOSAMINOGLYCAN COMPLEXES FROM HUMAN ATHEROSCLEROTIC PLAQUES1976
- Enzymatic Methods for the Determination of Small Quantities of Isomeric Chondroitin SulfatesJournal of Biological Chemistry, 1968
- Quantitative studies on the complexes formed between aortic mucopolysaccharides and serum lipoproteinsBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1967
- A study of the immunohistochemical localization of serum lipoproteins and other plasma proteins in human atherosclerotic lesionsExperimental and Molecular Pathology, 1965
- Mucopolysaccharides in the atheromatous aortaThe Journal of Pathology and Bacteriology, 1962
- The histogenesis of the atherosclerotic “fatty streak”Journal of Atherosclerosis Research, 1961
- THE ROLE OF MUCOPOLYSACCHARIDES IN THE PATHOGENESIS OF INTIMAL FIBROSIS AND ATHEROSCLEROSIS OF THE HUMAN AORTA1953
- Histogenesis of Coronary ArteriosclerosisCirculation, 1952