Control of Seed Germination by Abscisic Acid

Abstract

Germination of rape (B. napus L.) seeds proceeds in 2 phases, an initial imbibition phase and a subsequent growth phase. The time courses of water uptake, O2 uptake and ATP accumulation demonstrate that exogenous abscisic acid (ABA, 0.1 mmol/l) specifically prevents the embryo from entering the growth phase. The inhibition of water uptake by ABA is a rapid (lag-phase about 1 h) and fully reversible process which apparently is the cause rather than the result of changes of the energy metabolism. In untreated seeds, an osmotic pressure (polyethylene glycol 6000) of 11 bars is required for a simulation of the ABA effect on water uptake. However, in ABA-treated seeds an osmotic pressure of only 3 bars is sufficient to suppress water uptake. Thus, ABA lowers the ability of the embryo to absorb water under osmotic stress. In a 2-factor analysis of the simultaneous action of ABA and osmoticum on germination, a complete synergistic interaction between these factors was found while ABA and cycloheximide exhibit independent (multiplicative) coaction. These results are interpreted in terms of a common controlling point of ABA and osmotic stress in the water relations of germinating seeds.