Total, Phospholipid and Labile Phosphorus in Serum and Tissue Employing Chloric Acid and N-Phenyl-P-Phenylenediamine.

Abstract

A method is described for determination of serum and tissue total, phospholipid and hydrolyzable phosphorus using chloric acid digestion and subsequent formation and reduction of a phosphomolybdate complex with N-phenyl-P-phenylenediamine. This method compares well with established methods and possesses the advantages of ease of digestion color development and handling and stability of color complex.