Sulfated Trihexosylceramide from Gastric Mucosa Containing N-Acetylglucosamine

Abstract
A new sulfated glycosphingolipid containing N-acetylglucosamine was isolated from hog gastric mucosa by extraction with sodium acetate. Based on the results of partial acid hydrolysis, sequential degradation with specific glycosidases and methylation analysis of the native and desulfated compound, the structure of this glycolipid is proposed to be: SO3H (.fwdarw. 6) GlCNAc-(.beta.1 .fwdarw. 3)Gal(.beta.1 .fwdarw. 4)Glc(1 .fwdarw. 1)Cer (GlcNAc, N-acetylglucosamine; Cer, ceramide).