Cryopreparation of mammalian tissue for X‐ray microanalysis in STEM
- 1 February 1982
- journal article
- Published by Wiley in Journal of Microscopy
- Vol. 125 (2), 149-156
- https://doi.org/10.1111/j.1365-2818.1982.tb00332.x
Abstract
A cryopreparation technique for studies of ultrastructure and distribution of diffusible elements in biological tissue is described. Electron microscopical contrast and characteristic X-ray spectra are found to be poor in completely frozen-hydrated ultrathin cryosections of fresh chemically untreated tissue. Both STEM contrast and detection of characteristic X-rays are enhanced by careful freeze-drying in the microscope. Although the ultrastructure is affected by ice crystals, intracellular compartments can be identified by STEM without staining and studied by X-ray microanalysis.Keywords
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