Production of recombinant sarcotoxin IA in Bombyx mori cells
- 15 December 1990
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 272 (3), 633-636
- https://doi.org/10.1042/bj2720633
Abstract
A cDNA for sarcotoxin IA, an antibacterial protein of Sarcophaga peregrina (fleshfly), was inserted into a silkworm baculovirus vector and expressed in Bm-N cells, a line of Bombyx mori cells. When a cysteine proteinase inhibitor, p-chloromercuribenzenesulphonic acid, was present in the culture medium, a significant amount of recombinant sarcotoxin IA accumulated, but without this reagent the product seemed to be degraded in this system. The C-terminus of the recombinant sarcotoxin IA seemed to be glycine, not amidated arginine as found in authentic sarcotoxin IA. Probably, Bm-N cells lack the C-terminal alpha-amidation enzyme.Keywords
This publication has 14 references indexed in Scilit:
- Primary structure of sarcotoxin I, an antibacterial protein induced in the hemolymph of Sarcophaga peregrina (flesh fly) larvae.Journal of Biological Chemistry, 1985
- Production of human α-interferon in silkworm using a baculovirus vectorNature, 1985
- Molecular cloning, cDNA sequencing, and chemical synthesis of cecropin B from Hyalophora cecropia.Proceedings of the National Academy of Sciences, 1985
- Characterization of a peptide alpha-amidation activity from rat anterior pituitary.Journal of Biological Chemistry, 1984
- Purification and characterization of an antibacterial protein from haemolymph of Sarcophaga peregrina (flesh-fly) larvaeBiochemical Journal, 1983
- Measurement of Sarcophaga peregrina lectin under various physiological conditions by radioimmunoassay.Journal of Biological Chemistry, 1983
- Insect Immunity: Isolation and Structure of Cecropins B and D from Pupae of the Chinese Oak Silk Moth, Antheraea pernyiEuropean Journal of Biochemistry, 1982
- Insect Immunity. Purification and Properties of Three Inducible Bactericidal Proteins from Hemolymph of Immunized Pupae of Hyalophora cecropiaEuropean Journal of Biochemistry, 1980
- The gastrointestinal stimulus to insulin releaseJCI Insight, 1969
- STUDIES ON HOST-VIRUS INTERACTIONS IN THE CHICK EMBRYO-INFLUENZA VIRUS SYSTEMThe Journal of Experimental Medicine, 1953