Separation of Enantiomers in Ion-Pair Chromatographic Systems

Abstract
Enantiomers of amines and acids have been separated in ion-pair chromatographic systems with chiral agents present in mobile or stationary phase. Chiral counter ions such as quinine or camphorsulfonate have been used in liquid solid systems with organic mobile phases, while the chiral complexing agents albumin and (+)-n-dibutyltartrate have been used in reversed phase systems. Regulation of retention and stereoselectivity as well as improvement of detection sensitivity by the composition of the system is discussed.