Studies on the Action of Biogenic Amines on Cockroach Heart
Open Access
- 1 April 1977
- journal article
- research article
- Published by The Company of Biologists in Journal of Experimental Biology
- Vol. 67 (1), 1-15
- https://doi.org/10.1242/jeb.67.1.1
Abstract
Measurement of the beat of the semi-isolated cockroach heart, by impedance conversion, showed that the whole abdominal heart of large nymphs or adults responded to low concentrations of acetylcholine (ACh) or 5-hydroxytryptamine (5-HT) by increasing the rate of heartbeat. Preparations, which included 2-chamber sections of the abdominal heart, showed vastly reduced responses to ACh compared to the responses of the whole abdominal heart. Responses to 5-HT were similar in both preparations. Following assay with 5-HT, the cockroach heartbeat often developed marked regularity. ACh assays produced such regularity only rarely. The sectioned cockroach heart preparation was decreasingly responsive to 5-HT > synephrine > octopamine > tryptamine > dopamine > tyramine. Dose-response curves revealed that the antagonist 501 c interacted competitively with 5-HT on the cockroach heart preparation. 501 c appeared to be a non-competitive antagonist to octopamine, suggesting that 5-HT and octopamine act at separate receptor sites on the myocardium. Experiments in which solutions of dibutyryl cyclic AMP, dibutyryl cyclic GMP, or aminophylline were continuously perfused on the cockroach heart, failed to establish that cyclic nucleotides mediate neurotransmitter or hormone action on this tissue. Cockroach hearts were not responsive to prostaglandin E1, F1, picrotoxin, L-glutamate, or ATP below 10−3-10−4M doses.This publication has 17 references indexed in Scilit:
- Calcium and cyclic AMP as possible mediators of neurohormone action in the hindgut of the cockroach, Leucophaea maderaeJournal of Insect Physiology, 1975
- Neurosecretion and the Control of Visceral Organs in InsectsAnnual Review of Entomology, 1975
- FACTORS AFFECTING HEART RATES IN INSECTSPublished by Elsevier ,1974
- Experimentelle Untersuchungen zum Freisetzungsmechanismus von Neurohormonen nach elektrischer Reizung der Corpora cardiaca von Periplaneta americana in vitroJournal of Insect Physiology, 1972
- Transmitter substances in insects: A reviewComparative and General Pharmacology, 1971
- Stimulation of movements of Malpighian tubules of Carausius by pharmacologically active substances and tissue extractsJournal of Insect Physiology, 1971
- Cyclic Adenosine Monophosphate: Possible Mediator for Norepinephrine Effects on Cerebellar Purkinje CellsScience, 1969
- The pharmacology of cardioaccelerators in the central nervous system of Periplaneta americana (L.)Comparative Biochemistry and Physiology, 1969
- COCKROACH HEART RESPONSE TO CARDIO‐ACCELERATORS*Entomologia Experimentalis et Applicata, 1969
- New Peripheral Antagonists of 5-HydroxytryptamineNature, 1967