Co-Stimulation with anti-CD28 (Kolt-2) enhances DNA synthesis by defective T cells in common variable immunodeficiency
- 1 February 1994
- journal article
- Published by Oxford University Press (OUP) in Clinical and Experimental Immunology
- Vol. 95 (2), 204-208
- https://doi.org/10.1111/j.1365-2249.1994.tb06511.x
Abstract
SUMMARY: In normal T cells, an ann-CD28 MoAb (Kolt-2) will synergize with the mitogenic stimuli Phytohaemagglutinin (PHA). anti-CD3 (OKT3) or a combination of anti-CD2 antibodies (OKT11 and GT2) in the induction of DNA synthesis. A subgroup of patients with common variable immunodeficiency (CVID) show a defect in DNA synthesis by T cells stimulated in vitro with the above mitogens. We have now investigated whether anti-CD28 will correct the defect. This strategy partially restored DNA synthesis, providing evidence that the CD28 co-stimulatory pathway in CVID T cells is normal. Ligation of CT28 acts through co-stimulating IL-2 secretion. The natural ligand (B7) for CD28 on antigen-presenting cells from CVID patients is expressed normally. We conclude that the defect in CVID T cells lies in pathways that lead to transcription of the IL-2 gene other than that induced by ligation of CD2K with Kolt-2.Keywords
This publication has 23 references indexed in Scilit:
- The Role of the CD28 Receptor During T Cell Responses to AntigenAnnual Review of Immunology, 1993
- The AP-1 site at -150 bp, but not the NF-kappa B site, is likely to represent the major target of protein kinase C in the interleukin 2 promoter.The Journal of Experimental Medicine, 1992
- Abnormalities in CD4+ T lymphocyte subsets in patients with common variable immunodeficiencyClinical Immunology and Immunopathology, 1991
- Defects in proliferative responses of T cells from patients with common variable immunodeficiency on direct activation of protein kinase CClinical and Experimental Immunology, 1991
- Role of interleukin-2 and interleukin-6 in the mitogen responsiveness of T cells from patients with ‘common-variable’ hypogammaglobulinaemiaClinical and Experimental Immunology, 1990
- Classification of patients with common variable immunodeficiency by B cell secretion of IgM and IgG in response to anti-IgM and interleukin-2Clinical Immunology and Immunopathology, 1990
- Clonal Expansion Versus Functional Clonal Inactivation: A Costimulatory Signalling Pathway Determines the Outcome of T Cell Antigen Receptor OccupancyAnnual Review of Immunology, 1989
- Clinical and immunologic analyses of 103 patients with common variable immunodeficiencyJournal of Clinical Immunology, 1989
- T-cell activation defect in common variable immunodeficiency: Restoration by phorbol myristate acetate (PMA) or allogeneic macrophagesClinical Immunology and Immunopathology, 1987
- A 45‐kDa human T‐cell membrane glycoprotein functions in the regulation of cell proliferative responsesEuropean Journal of Biochemistry, 1984