Induction of Immunity to a Mouse Lymphoma by Multiple Methods, Including Vaccination With Soluble Membrane Fractions2

Abstract

6C3HED lymphoma grows to the point of lethality from a small inoculum in C3H mice, the strain of origin. Noncytotoxic antibodies that form during tumor growth give evidence of an immune response by the host. C3H mice were rendered immune to 6C3HED by 1) L-asparaginase treatment of tumorbearing animals, 2) vaccination with iodoacetamide- modified 6C3HED cells, 3) transfer of spleen and lymph node cells from immune mice (100% protection at a lymphoid cell: tumor cell ratio of 100 : 1), 4) preincubation of tumor cells with noncytotoxic immune serum before injection into nonimmune hosts, and 5) vaccination with soluble 6C3HED membrane antigen(s). Two membrane fractions isolated from Sephadex G-200 columns contained antigens that were effective in protective vaccination and in neutralizing antibody in the highly specific C3H anti-6C3HED.