Factor V anticoagulants: clinical, biochemical, and immunological observations
Open Access
- 1 August 1970
- journal article
- Published by American Society for Clinical Investigation in JCI Insight
- Vol. 49 (8), 1578-1588
- https://doi.org/10.1172/jci106375
Abstract
A patient who had received multiple transfusions for complications of acute hemorrhagic pancreatitis developed a potent factor V anticoagulant with bleeding due to defective hemostasis. Despite its potency, the anticoagulant disappeared within 15 days of its first manifestation. A second patient with adenocarcinoma of the colon developed an anticoagulant to factor V postoperatively after a single blood transfusion. The anticoagulants appeared to react stoichiometrically with factor V in normal plasma in vitro. They had the physicochemical properties of immunoglobulins, and their activity was neutralized by antihuman immunoglobulin antiserum. One anticoagulant appeared to be slightly more active against homologous than against autologous factor V, but it also inhibited heterologous factor V. Both anticoagulants progressively inactivated intrinsic prothrombin activator formed from normal reagents in the incubation mixture of the thromboplastin generation test, thus confirming that factor V is required for the effective action of the intrinsic prothrombin activator. Since the anticoagulants were immunoglobulins whose activity was consumed in their reaction with factor V, consumption of anticoagulant activity was used to detect factor V antigenic material in test materials. Human serum without factor V clotting activity was found to consume anticoagulant activity, i.e., to contain inactive factor V antigenic material. Plasma from two patients with hereditary factor V deficiency (parahemophilia) failed to consume significant anticoagulant activity. Thus, the lack of factor V activity in these patients represents a deficiency of factor V molecules rather than the synthesis of a defective molecule with impaired clotting activity.Keywords
This publication has 31 references indexed in Scilit:
- A Specific Inhibitor of Human Clotting Factor VActa Haematologica, 1968
- Studies on the formation of the prothrombin-converting complexBiochemical Journal, 1967
- The Immunologic Character of Acquired Inhibitors of Antihemophilic Globulin (Factor VIII) and the Kinetics of Their Interaction with Factor VIII*JCI Insight, 1967
- Post-Transfusion PurpuraNew England Journal of Medicine, 1966
- Starch Block Electrophoresis of Plasma and Serum Clotting Factors. Separation of Activated PTC (PTC’)Blood, 1965
- Observations on the interaction of phospholipids and certain clotting factors in prothrombin activator formationBiochimica et Biophysica Acta (BBA) - General Subjects, 1964
- The Partial Thromboplastin Time with Kaolin: A Simple Screening Test for First Stage Plasma Clotting Factor DeficienciesAmerican Journal of Clinical Pathology, 1961
- Human Gamma Globulin Fractionation on Anion Exchange Cellulose ColumnsJournal of Biological Chemistry, 1959
- The Preparation of Proaccelerin Deficient (Parahemophilia) Plasma for the Assay of ProaccelerinScandinavian Journal of Clinical and Laboratory Investigation, 1957
- Zone ElectrophoresisPublished by Wiley ,1954