Paper Chromatography of the Amino-Acids in Horseradish Peroxidase.
- 1 January 1950
- journal article
- research article
- Published by Danish Chemical Society in Acta Chemica Scandinavica
- Vol. 4 (3), 508-511
- https://doi.org/10.3891/acta.chem.scand.04-0508
Abstract
The protein portion of horseradish peroxidase was hydrolyzed and separated into acid, basic and neutral amino acids by means of a micro-electrodialysis procedure. The fractions were chromatographed by a slight modification of the ascending method of Williams and Kirby (Science 107: 481, 1948). Qualitatively, aspartic, glutamic, and cysteic acids, arginine, histidine, lysine, alanine, glycine, hydroxyproline, leucine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine and valine were identified.Keywords
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