Positive and negative regulatory elements control the expression of the UGA4 gene coding for the inducible 4‐aminobutyric‐acid‐specific permease in Saccharomyces cerevisiae
- 1 May 1989
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 181 (2), 357-361
- https://doi.org/10.1111/j.1432-1033.1989.tb14732.x
Abstract
In Saccharomyces cerevisiae, the pathway of 4-aminobutyric acid catabolism, for use as a nitrogen source, involves a specific permease (encoded by the UGA4 gene) and two enzymes (encoded by the UGA1 and UGA2 genes, respectively). The synthesis of these proteins is induced by 4-aminobutyrate. It also requires the product of the UGA3 gene. Here, we describe four additional regulatory mutations which provide evidence for the existence of both positive and negative regulatory elements which control the final expression of the UGA4 gene. Some of them simultaneously control the expression of the UGA1 and UGA2 genes. Three classes of mutant with a constitutive 4-aminobutyrate-specific permease have been isolated. (a) Recessive mutations in the UGA43 gene suggest that the product of the UGA43 gene behaves like a trans-acting negative regulator of UGA4 gene expression. (b) The semi-dominant mutation (uga11), closely linked to the UGA4 gene, might affect the receptor of the UGA43 gene product. In these two classes of mutant, only the permease isconstitutive. (3) The uga81 mutation, closely linked to the UGA3 gene, makes the whole UGA regulon constitutive. On the other hand, recessive mutations at the UGA35 gene locus lead to non-inducibility of the UGA regulon. Hence the UGA35 gene product behaves like a second trans-acting positive regulator in addition to UGA3.This publication has 13 references indexed in Scilit:
- Activation of the yeast HO gene by release from multiple negative controlsCell, 1987
- Mutations affecting the enzymes involved in the utilization of 4‐aminobutyric acid as nitrogen source by the yeast Saccharomyces cerevisiaeEuropean Journal of Biochemistry, 1985
- A SUPPRESSOR OF snf1 MUTATIONS CAUSES CONSTITUTIVE HIGH-LEVEL INVERTASE SYNTHESIS IN YEASTGenetics, 1984
- A cis-dominant regulatory mutation linked to the argB-argC gene cluster in Saccharomyces cerevisiaeJournal of Molecular Biology, 1980
- Constitutive synthesis of the GAL4 protein, a galactose pathway regulator in saccharomyces cerevisiaeCell, 1979
- Specific induction of catabolism and its relation to repression of biosynthesis in arginine metabolism of Saccharomyces cerevisiaeJournal of Molecular Biology, 1978
- SaccharomycesPublished by Springer Nature ,1974
- Multiplicity of the amino acid permeases in Saccharomyces cerevisiaeBiochimica et Biophysica Acta (BBA) - General Subjects, 1966
- GENETICAL MUTANTS INDUCED BY ETHYL METHANESULFONATE INSACCHAROMYCESCanadian Journal of Genetics and Cytology, 1965