RNA Metabolism of Circulating Lymphocytes Studied in Man After Autotransfusion and in vitro 3H-Cytidine Labeling

Abstract

The labeling pattern of in vitro ³H-cytidine labeled blood lymphocytes was studied after autotransfusion in 2 hematologically normal patients and in 6 patients with untreated chronic lymphocytic leukemia. An in vitro incubation time of 45 min results in a rapid loss of label during the first hours, whereas after a 4 h incubation time the label is much more stable. The results are taken to indicate that during a 45 min incubation time predominantly metabolically unstable RNA-fractions become labeled, while after a 4 h in vitro incubation period a larger proportion of the stable RNA-fractions is labeled