Production of Chemotactic Activity by Guinea Pig Immunoglobulins Following Activation of the C3 Complement Shunt Pathway

Abstract

Guinea pig γ1 and γ2 immunoglobulins consume late C components (C3 to C9) when incubated with C4 deficient guinea pig serum. The γ2 antibodies produce greater hemolytic activity losses in normal than in C4 deficient guinea pig serum. In contrast, consumption of late component activity by the γ1 antibodies is similar in serum containing or lacking C4. Both types of immunoglobulins produce a serumderived factor chemotactic for rabbit polymorphonuclear leukocytes in either normal or C4 deficient serum. This factor has been identified as a cleavage product of C5.