Interaction of aminoacyl-tRNA synthetases and tRNA: Positive and negative cooperativity of their active centres
- 1 July 1976
- journal article
- research article
- Published by Springer Nature in Molecular Biology Reports
- Vol. 2 (6), 445-454
- https://doi.org/10.1007/bf00356933
Abstract
The influence of tRNA on the kinetics of PP-ATP exchange and aminoacyl-tRNA formation catalysed by leucyl-, phenylalanyl-, and tryptophanyl-tRNA synthetases has been investigated. These enzymes were chosen because they belong to three main classes of quaternary structure α1, α2β2 and α2, respectively. The present paper shows that the investigated synthetases manifest kinetic cooperativity of the active centres which is negative in the case of AAA formation and positive in the case of leucyl- and tryptophanyl-tRNA synthesis. The obtained data were interpreted with the aid of the trigger model of the enzyme.This publication has 18 references indexed in Scilit:
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