Members of several gene families influence survival of rat motoneurons in vitro and in vivo
- 15 December 1993
- journal article
- research article
- Published by Wiley in Journal of Neuroscience Research
- Vol. 36 (6), 663-671
- https://doi.org/10.1002/jnr.490360607
Abstract
The survival and functional maintenance of spinal motoneurons, both during the period of developmental cell death and in adulthood, have been shown to be dependent on trophic factors. In vitro experiments have previously been used to identify several survival factors for motoneurons, including CNTF, LIF, and members of the neurotrophin, FGF, and IGF gene families. Some of these factors have also been shown to be active in vivo, either on chick motoneurons during embryonic development or on lesioned facial and spinal motoneurons of the newborn rat. Here we demonstrate that lesioned newborn rat facial motoneurons can be rescued by NT-4/5, IGF-I and LIF. Furthermore, in contrast to chick motoneurons, the survival of isolated embryonic rat motoneurons can be maintained by the neurotrophins BDNF, NT-3, and NT-4/5. IGF-I and FGF-5 were also active in this system, each supporting more than 50% of the originally plated neurons. The responsiveness of motoneurons to multiple factors in vitro and in vivo suggests that motoneuron survival and function are regulated by the coordinated actions of members of different gene families.Keywords
This publication has 72 references indexed in Scilit:
- Cell interactions that determine sympathetic neuron transmitter phenotype and the neurokines that mediate themJournal of Neurobiology, 1993
- Injury‐induced Regulation of Ciliary Neurotrophic Factor mRNA in the Adult Rat BrainEuropean Journal of Neuroscience, 1993
- Insulin-like growth factor binding proteins of equine serumBiochemical and Biophysical Research Communications, 1992
- Cytokines and neurokines: related ligands and related receptorsTrends in Neurosciences, 1992
- Effects of ciliary neuronotrophic factor on rat spinal cord neurons in vitro: survival and expression of choline acetyltransferase and low-affinity nerve growth factor receptorsDevelopmental Brain Research, 1991
- Receptor Binding, Endocytosis, and Mitogenesis of Insulin‐Like Growth Factors I and II in Fetal Rat Brain NeuronsJournal of Neurochemistry, 1991
- The cholinergic neuronal differentiation factor from heart cell conditioned medium is different from the cholinergic factors in sciatic nerve and spinal cordDevelopmental Biology, 1990
- Growth hormone-dependent insulin-like growth factor (IGF) binding protein both inhibits and potentiates IGF-I-stimulated DNA synthesis in human skin fibroblastsBiochemical and Biophysical Research Communications, 1988
- Motoneurone survival requirements during development: the change from immature astrocyte dependence to myotube dependenceDevelopmental Brain Research, 1986
- Motoneurone survival is induced by immature astrocytes from developing avian spinal cordDevelopmental Brain Research, 1985