Horseradish Peroxidase Enzyme Electrodes for Nadh and Nadph
- 31 December 1981
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 15 (18), 1479-1491
- https://doi.org/10.1080/00032718208065139
Abstract
The aerobic oxidation of reduced pyridine phosphonucleotides catalyzed by horseradish peroxidase (E.C.1.11.1.7) (HRP) in combination with Mn2+-ions, phenolics and redox dyes was used to perform NADH and NADPH determinations with an amperometric HRP enzyme electrode. Optimal operational conditions have been elaborated for such a sensor and a linear relationship between derivative current (dI/dt) and NADH-concentration was obtained between 2.10−5 and at least 2.10−4 mol/l. The coefficient of variation was equal to or smaller than 4 %. The time for one measurement was less than 10 minutes including equilibration of the electrode.Keywords
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