Tetanus Toxin Inhibition of K+ ‐Stimulated [3H]GABA Release from Developing Cell Cultures of the Rat Cerebellum
- 1 March 1983
- journal article
- Published by Wiley in Journal of Neurochemistry
- Vol. 40 (3), 887-890
- https://doi.org/10.1111/j.1471-4159.1983.tb08064.x
Abstract
The effect of tetanus toxin pretreatment on K+-stimulated [3H]gamma-aminobutyric acid release from neuron-enriched cerebellar cell cultures at various stages during their development in vitro was assessed. Tetanus toxin had little inhibitory effect on immature (1-3-day-old) cultures, but markedly reduced K+-evoked [3H]gamma-aminobutyric acid release from 7- and 14-day-old cultures (approximately 80% inhibition). It is suggested that cerebellar neurons in culture develop tetanus toxin-sensitive transmitter release mechanisms similar to their in vivo counterparts.Keywords
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