Retrieving vesicles in secretion-induced rat chromaffin cells contain fodrin.
Open Access
- 1 November 1989
- journal article
- research article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 37 (11), 1589-1599
- https://doi.org/10.1177/37.11.2509551
Abstract
We examined the distribution of fodrin and cytochrome b561 in secretion-induced rat chromaffin cells (epinephrine cells) by immunofluorescence and immunoelectron microscopy. Fasted rats injected with a large dose of insulin were perfusion-fixed and frozen sections of the adrenal medulla were immunolabeled. Fodrin, a peripheral membrane protein, was distributed only in the cell periphery in control cells, but was observed in the cell interior after the insulin treatment; many of the markers were found around small vesicles, 50-200 nm in diameter, and large vacuoles, more than 500 nm in diameter. On the other hand, cytochrome b561, an integral membrane protein, was seen only in the chromaffin granules in control cells, and appeared in small vesicles in the stimulated cells but not in large vacuoles. By double immunolabeling it was shown that cytochrome b561 coexisted with fodrin in the small vesicles. The coexistence of the two proteins was confirmed by the labeling of subcellular particles immunoadsorbed from the insulin-treated adrenal medulla homogenate; vesicles immunoisolated with anti-fodrin antibody on polyacrylamide beads were positively immunolabeled with anti-cytochrome b561 antibody. The present results show that during massive secretion fodrin is taken into the cell interior by vesicles, which may be a mechanism that retrieves the secretory granule membrane from the cell surface.This publication has 12 references indexed in Scilit:
- Immunocytochemical studies of endothelial cells in vivo. I. The presence of desmin only, or of desmin plus vimentin, or vimentin only, in the endothelial cells of different capillaries of the adult chicken.The Journal of cell biology, 1986
- Exocytotic exposure and retrieval of membrane antigens of chromaffin granules: quantitative evaluation of immunofluorescence on the surface of chromaffin cells.The Journal of cell biology, 1984
- Fodrin is the general spectrin-like protein found in most cells whereas spectrin and the TW protein have a restricted distributionCell, 1983
- Nonerythrocyte spectrins: actin-membrane attachment proteins occurring in many cell typesThe Journal of cell biology, 1982
- Widespread occurrence of avian spectrin in nonerythroid cellsCell, 1982
- Exocytosis and membrane recyclingPhilosophical Transactions of the Royal Society of London. B, Biological Sciences, 1981
- Endocytosis in secretory cellsPhilosophical Transactions of the Royal Society of London. B, Biological Sciences, 1981
- Coated pits act as molecular filters.Proceedings of the National Academy of Sciences, 1980
- Immunochemistry on ultrathin frozen sectionsJournal of Molecular Histology, 1980
- Functional and morphological characterization of isolated bovine adrenal medullary cells.The Journal of cell biology, 1978