Characterization of lactose transport in Kluyveromyces lactis
- 1 June 1983
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 154 (3), 1245-1251
- https://doi.org/10.1128/jb.154.3.1245-1251.1983
Abstract
Lactose uptake in K. lactis is mediated by an inducible transport system. Induction, elicited by lactose or galactose, of the transporter required protein synthesis. Transport of lactose required an energy-generating system and occurred by an active process, since an intracellular lactose concentration 175 times greater than the extracellular concentration could be obtained. The Km for lactose transport was about 2.8 mM in uninduced and lactose- or galactose-induced cells. The lactose transporters in K. lactis and Escherichia coli appear to be different since they respond uniquely to inhibition by substrate analogs.This publication has 15 references indexed in Scilit:
- Genetic regulation: yeast mutants constitutive for beta-galactosidase activity have an increased level of beta-galactosidase messenger ribonucleic acid.Molecular and Cellular Biology, 1981
- Transcriptional regulation of the Kluyveromyces lactis beta-galactosidase gene.Molecular and Cellular Biology, 1981
- LAC4 IS THE STRUCTURAL GENE FOR BETA-GALACTOSIDASE IN KLUYVEROMYCES-LACTIS1981
- Mutations affecting synthesis of beta-galactosidase activity in the yeast Kluyveromyces lactis.1980
- Physiological Studies of β-Galactosidase Induction in Kluyveromyces lactisJournal of Bacteriology, 1980
- Dierect measurement of the binding of labeled sugars to the lactose permease M protein.1974
- Direct Measurement of the Binding of Labeled Sugars to the Lactose Permease M ProteinPublished by Elsevier ,1974
- lac repressor-operator interactionJournal of Molecular Biology, 1972
- The Statistical Analysis of Enzyme Kinetic DataPublished by Wiley ,1967
- A simple procedure for the preparation of uridine diphosphogalactoseAnalytical Biochemistry, 1961