The eggs were centrifuged in cane sugar solns., raffinose solns., and within the jelly inside the capsule. The polar end of the egg becomes separated from the rest of the egg (bottoms). These polar portions may be very small, containing only oil, or as large as half the egg. Some of the bottoms contain no chromatin, it having been driven into the top. These bottoms undergo rhythmic changes comparable to the changes in this region when it is a part of the egg, i.e., the lobe appears twice. The results are compared with the changes in shape that the third antipolar lobe undergoes when isolated. It may also be without chromatin. It is suggested that the changes in the latter are more comparable to the progressive changes the third lobe undergoes when part of the egg, rather than a retrogressive change in the reappearance of the earlier lobes, while the changes in shape of the isolated third lobe are more nearly comparable to the progressive changes that take place when it remains a part of one of the blastomeres.