Characterization of inl + transformants of Neurospora crassa obtained with a recombinant cosmid-pool
Open Access
- 1 November 1986
- journal article
- research article
- Published by Springer Nature in Current Genetics
- Vol. 11 (2), 131-137
- https://doi.org/10.1007/bf00378205
Abstract
We constructed a Neurospora crassa gene library in a cosmid vector and used the cosmid-pool DNA to transform an inl, rg Neurospora crassa strain to inositol prototrophy. The inl+ colonies obtained in this experiment proved to be integrative type transformants. Genetic analysis revealed that the integration event occurred at or near the inl locus. In one of the transformants the inl+ trait exhibited mitotic and meiotic instability. In hybridization experiments free plasmids were detected in the F1 progeny of the transformants. We were able to recover eleven different plasmids from the F1 progeny of the transformants. None of these plasmids proved to carry a functional copy of the inl+ gene as judged by its transforming ability. Possible explanations for the observed phenomena are discussed.Keywords
This publication has 29 references indexed in Scilit:
- A recombinant plasmid carrying the mitochondrial plasmid sequence of Neurospora intermedia LaBelle yields new plasmid derivatives in Neurospora crassa transformantsCurrent Genetics, 1985
- Possible role of a regulatory gene product upon the myo-inositol-1-phosphate synthase production in Neurospora crassaBiochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1983
- Molecular cloning and expression in Escherichia coli of two modification methylase genes of Bacillus subtilisGene, 1983
- Isolation of a gene from Drosophila by complementation in yeastNature, 1980
- Characterization of the sequence complexity and organization of the Neurospora crassa genomeBiochemistry, 1979
- Molecular cloning of the DNA ligase gene from bacteriophage T4Journal of Molecular Biology, 1979
- The isolation of structural genes from libraries of eucaryotic DNACell, 1978
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977
- A colony bank containing synthetic CoI EI hybrid plasmids representative of the entire E. coli genomeCell, 1976
- A complementation analysis of the restriction and modification of DNA in Escherichia coliJournal of Molecular Biology, 1969