Isolation of Human Urinary Kallikrein by Affinity Chromatography. Determination of human urinary kallikrein, I.
- 1 January 1977
- journal article
- research article
- Published by Walter de Gruyter GmbH in cclm
- Vol. 15 (1-12), 479-484
- https://doi.org/10.1515/cclm.1977.15.1-12.479
Abstract
Human urinary kallikrein (EC 3.4.21.8) was purified to electrophoretic homogeneity by gel filtration on Sephadex G-100, followed by affinity chromatography on aprotinin-Sepharose. The MW, estimated by gel filtration, is 64,000 daltons, by dodecyl sulfate electrophoresis 45,000 daltons and 29,000 daltons, and it is microheterogeneous on electrofocusing, yielding isoelectric point values of 3.8, 3.9 and 4.05. The kinetic constants for hydrolysis of substrates and human HMW[high molecular weight]-kininogen were determined. Benzamidine and aprotinin are competitive inhibitors of human urinary kallikrein.This publication has 27 references indexed in Scilit:
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