Metabolism of propionate by sheep liver. Stimulation of the mitochondrial rate by factors from the cell sap
- 1 May 1965
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 95 (2), 423-430
- https://doi.org/10.1042/bj0950423
Abstract
1. The rate of metabolism of propionate by aged sheep-liver mitochondria in the presence of oxygen + carbon dioxide (95:5) was 5.0 (+/- s.e.m. 0.8) mumoles/mg. of mitochondrial N/hr. 2. When aged in the presence of the mitochondrial supernatant the rate was increased. Mitochondria from 0.33g. of liver, when combined with the corresponding mitochondrial supernatant from 0.08g. of liver, metabolized propionate at a rate of 11.4 (+/- s.e.m. 1.2) mumoles/mg. of mitochondrial N/hr. This rate is comparable with rates previously obtained with aged nuclear-free homogenates. 3. Two factors in the mitochondrial supernatant were detected, which when combined reproduced the effect of the fresh supernatant and prevented loss of activity on aging. One of these was non-diffusible and was recovered by fractionation of the dialysed mitochondrial supernatant with ammonium sulphate. The second factor was present in an ultrafiltrate of fresh mitochondrial supernatant and in boiled mitochondrial supernatant; it was isolated and identified as l(+)-glutamate. 4. The effect of the non-diffusible factor was due to protection of the mitochondria from the aging process, whereas glutamate served both in this capacity and as a direct stimulant of propionate metabolism at low concentration.This publication has 9 references indexed in Scilit:
- METABOLISM OF PROPIONATE BY SHEEP LIVER. OXIDATION OF PROPIONATE BY HOMOGENATESBiochemical Journal, 1965
- METABOLISM OF PROPIONATE BY SHEEP LIVER. INTERRELATIONS OF PROPIONATE AND GLUTAMATE IN AGED MITOCHONDRIABiochemical Journal, 1965
- [Free amino acids and polypeptides in biological fluids and tissues. IX. Combined forms of amino acids with an acid character].1962
- Comparative Ultrafiltration of Calcium and Strontium in Serum.Experimental Biology and Medicine, 1958
- Appendix—A simply constructed tissue homogenizerBiochemical Journal, 1956
- A nomogram for ammonium sulphate solutionsBiochemical Journal, 1953
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951
- PHOTOMETRIC NINHYDRIN METHOD FOR USE IN THE CHROMATOGRAPHY OF AMINO ACIDSJournal of Biological Chemistry, 1948
- Separation of acidic amino-acids by means of a synthetic anion exchange resinBiochemical Journal, 1948