FAILURE OF ZONA PENETRATION OF HAMSTER SPERMATOZOA AFTER PROLONGED PREINCUBATION IN A BLOOD SERUM FRACTION

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Abstract
Hamster spermatozoa preincubated in a human blood serum fraction for 2 to 3 hr fertilize over 90% of hamster eggs with intact zonae pellucidae. Spermatozoa preincubated for 4 to 6 hr lose the ability to cross the zona pellucida, but can enter the cytoplasm of zona-free eggs.