Stimulation of Chondroitin Sulfate Proteoglycan Production by Chondrocytes in Monolayer
- 1 January 1975
- journal article
- research article
- Published by Taylor & Francis in Connective Tissue Research
- Vol. 3 (2-3), 115-122
- https://doi.org/10.3109/03008207509152169
Abstract
Chondrocytes in monolayer undergo morphological and biochemical changes which culminate in the establishment of cartilage nodules in vitro. Chondroitin sulfate or heparin, added to the culture media of these cells, stimulates the production of chondroitin sulfate proteoglycan over the entire period of culture with a maximum effect during the log phase of growth. In addition, a lag of 2-3 hours is required before an increase in sulfate incorporation into polysaccharide is observed. The responsiveness of chondrocytes is influenced by several factors, such as cell density, conditioned media and enzyme treatment. Furthermore, puromycin abolishes the endogenous as well as the stimulated synthesis, demonstrating the necessity for core protein synthesis in both synthetic processes. Addition of beta-D-xylosides (which presumably act as initiators of chondroitin sulfate polysaccharide synthesis) and chondroitin sulfate, concurrently, stimulate sulfate incorporation to levels higher than either agent alone, indicating that these compounds act by different mechanisms.Keywords
This publication has 15 references indexed in Scilit:
- Stimulation of Synthesis of Free Chondroitin Sulfate Chains by β-D-Xylosides in Cultured CellsProceedings of the National Academy of Sciences, 1974
- Stimulation of Extracellular Matrix Synthesis in the Developing Cornea by GlycosaminoglycansProceedings of the National Academy of Sciences, 1974
- Environmental enhancement of in vitro chondrogenesisDevelopmental Biology, 1973
- Control of Chondrogenesis in Limb-Bud Cell Cultures by BromodeoxyuridineProceedings of the National Academy of Sciences, 1973
- INHIBITION OF CELLULAR DIFFERENTIATION BY PHOSPHOLIPASE CThe Journal of cell biology, 1973
- Stimulation of Chondromucoprotein Synthesis in Chondrocytes by Extracellular ChondromucoproteinProceedings of the National Academy of Sciences, 1972
- Synthesis of Acid Mucopolysaccharides by Glial Tumor Cells in Tissue CultureProceedings of the National Academy of Sciences, 1970
- A DIFFUSIBLE FACTOR WHICH SUSTAINS CONTACT INHIBITION OF REPLICATIONThe Journal of cell biology, 1969
- Hurler's syndrome: biosynthesis of acid mucopolysaccharides in tissue culture.Proceedings of the National Academy of Sciences, 1966
- The biosynthesis of chondroitin-sulfate protein complex.Proceedings of the National Academy of Sciences, 1965