Antigens of Cryptosporidium Sporozoites Recognized by Immune Sera of Infected Animals and Humans
- 1 February 1988
- journal article
- research article
- Published by JSTOR in Journal of Parasitology
- Vol. 74 (1), 135-143
- https://doi.org/10.2307/3282489
Abstract
The humoral response of humans, calves, and horses to Cryptosporidium sporozoite antigens was evaluated using a western blot technique. Sera from calves, humans, and horses were obtained at various times following the detection of infection. Sera were reacted with detergent-solubilized, sodium dodecyl sulfate polyacrylamide gel-electrophoresed (SDS-PAGE) sporozoite antigens. The number of antigens recognized by immune sera from humans and animals increased with time postinfection. A 20-kDa antigen appears to be a major sporozoite surface determinant labeled via membrane protein biotinylated and recognized by mouse monoclonal antibodies using indirect immunofluorescence and western blotting. Detectable recognition of the 20-kDa band occurred in 3-wk postinfection (PI) sera from all species tested. Reactivity to the 20-kDa band diminished significantly in sera 5 mo PI or longer from infected humans with no known recurrence of cryptosporidial diarrhea. In contrast, 12-mo PI sera from an individual constantly exposed to oocysts under working conditions was as strongly reactive as the 3-wk convalescent sera. Serum reactivity to the 20-kDa antigen appears to be a good indicator of exposure to Cryptosporidium.This publication has 15 references indexed in Scilit:
- The Effects of Reducing Conditions, Medium, pH, Temperature, and Time on in Vitro Excystation of CryptosporidiumThe Journal of Protozoology, 1984
- Cryptosporidiosis in Children from Some Highland Costa Rican Rural and Urban Areas *The American Journal of Tropical Medicine and Hygiene, 1984
- Development of Human and Calf Cryptosporidium in Chicken EmbryosThe Journal of Infectious Diseases, 1983
- Demonstration of serum antibodies to Cryptosporidium sp. in normal and immunodeficient humans with confirmed infectionsJournal of Clinical Microbiology, 1983
- Chronic malabsorption due to cryptosporidiosis in a child with immunoglobulin deficiencyGut, 1982
- Prevalence of Cryptosporidium Antibodies in 10 Animal SpeciesJournal of Clinical Microbiology, 1981
- Respiratory Cryptosporidiosis in Broiler ChickensAvian Diseases, 1981
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Cryptosporidial enteritis in a patient with congenital hypogammaglobulinemiaHuman Pathology, 1979
- Overwhelming Watery Diarrhea Associated with a Cryptosporidium in an Immunosuppressed PatientGastroenterology, 1976