Expression of the multidrug transporter, P-glycoprotein, in acute leukemia cells and correlation to clinical drug resistance
- 1 September 1990
Abstract
The overexpression of a cell-surface glycoprotein termed P-glycoprotein (P-gp) is frequently associated with multi-drug resistance (MDR) in cell lines in vitro. To evaluate the implications of P-gp expression in clinical drug resistance, the authors examined the expression of P-gp in leukemia cells from patients with acute myelogenous leukemia (AML) and those with acute lymphoblastic leukemia (ALL) at initial presentation and relapse, using immunoblotting with a monoclonal antibody against P-gp, C219. Nine of 17 patients with AML and four of 11 patients with ALL had P-gp-positive results at the initial presentation, and most P-gp-positive patients did not respond to chemotherapy. Four of seven patients at the relapsed stage and all three patients with preceding myelodysplastic syndrome had P-gp-positive results. The expression of P-gp and clinical refractoriness to chemotherapy were highly correlated. These data indicate that the expression of P-gp is closely related to clinical drug resistance in acute leukemia.This publication has 32 references indexed in Scilit:
- Multidrug resistance in haemopoietic cell lines, myelodysplastic syndromes and acute myeloblastic leukaemiaBritish Journal of Haematology, 1989
- Drug-resistance in multiple myeloma and non-Hodgkin's lymphoma: detection of P-glycoprotein and potential circumvention by addition of verapamil to chemotherapy.Journal of Clinical Oncology, 1989
- Expression of Multidrug Resistance Gene in Human CancersJNCI Journal of the National Cancer Institute, 1989
- MECHANISMS OF MULTIDRUG RESISTANCE AND IMPLICATIONS FOR THERAPYJapanese Journal of Cancer Research, 1988
- P-glycoprotein in human sarcoma: evidence for multidrug resistance.Journal of Clinical Oncology, 1987
- Expression of a multidrug-resistance gene in human tumors and tissues.Proceedings of the National Academy of Sciences, 1987
- Uptake and retention of adriamycin and daunorubicin by sensitive and anthracycline-resistant sublines of P388 leukemiaBiochemical Pharmacology, 1978
- A surface glycoprotein modulating drug permeability in Chinese hamster ovary cell mutantsBiochimica et Biophysica Acta (BBA) - Biomembranes, 1976
- Reduced permeability in CHO cells as a mechanism of resistance to colchicineJournal of Cellular Physiology, 1974
- Active outward transport of daunomycin in resistant ehrlich ascites tumor cellsBiochimica et Biophysica Acta (BBA) - Biomembranes, 1973