Enzyme-Linked Immunosorbent Assay for Detection of Rift Valley Fever Virus Antigen in Mosquitoes *
- 1 March 1985
- journal article
- research article
- Published by American Society of Tropical Medicine and Hygiene in The American Journal of Tropical Medicine and Hygiene
- Vol. 34 (2), 400-405
- https://doi.org/10.4269/ajtmh.1985.34.400
Abstract
An enzyme-linked immunosorbent assay (ELISA) was used to measure the quantity of Rift Valley fever (RVF) viral antigen in infected Egyptian Culex pipiens. Infectivity, as measured by plaque assay, was significantly correlated with viral antigen, as measured by the ELISA, in all groups of mosquitoes regardless of the time interval after the infectious blood meal. The proportion of noninfectious viral antigen in these groups increased with time. When individual mosquitoes were assayed the plaque assay and the ELISA techniques had similar sensitivity (100% vs. 93%, respectively) and specificity (94% vs. 94%, respectively) in detecting mosquitoes capable of transmitting virus to susceptible hamsters. The ELISA may be useful in detecting RVF-infected arthropods in the field because it provides a rapid, sensitive, and specific test.This publication has 4 references indexed in Scilit:
- Replication and Dissemination of Rift Valley Fever Virus in Culex PipiensThe American Journal of Tropical Medicine and Hygiene, 1984
- Detection of Rift Valley fever virus antigen by enzyme-linked immunosorbent assayJournal of Clinical Microbiology, 1983
- Dissemination Barriers for Western Equine Encephalomyelitis Virus in Culex Tarsalis Infected after Ingestion of Low Viral Doses *The American Journal of Tropical Medicine and Hygiene, 1981
- Experimental Transmission and Field Isolation Studies Implicating Culex Pipiens as a Vector of Rift Valley Fever Virus in EgyptThe American Journal of Tropical Medicine and Hygiene, 1980