Synthesis of methyl (R)- and (S)-[18O]phosphorothioates and determination of the absolute configuration at phosphorus of the diastereoisomers of adenosine 5′-(1-thiotriphosphate)

Abstract

A general route for the synthesis of enantiomeric [¹⁸O]phosphorothioate esters of known absolute configuration has been developed, and isomer A of adenosine 5′-(1-thiotriphosphate) shown to have the (S)-configuration at P_α.