Cell-Mediated Tumor Immunity Measured In Vitro and In Vivo With Soluble Tumor-Specific Antigens2

Abstract

Tumor-specific cellular immunity to lines of diethylnitrosamine-induced guinea pig hepatomas was demonstrated in vitro by macrophage migration inhibition and lymphocyte transformation, with water-soluble tumor antigens as the eliciting immunogens. Antigens From the ascites Form of line-1 and line-10 tumors were extracted with 3 M KCI and partially purified by ammonium sulfate precipitation. Peritoneal exudate cells From strain-2 guinea pigs immune to one or the other of the tumor cell lines were used For the in vitro studiesi a lymphocyte-rich subpopulation was used For lymphocyte transformation. Cellular responses to the solubilized tumor antigens were tumor line specific and dose dependent in both lymphocyte transformation and macrophage migration inhibition assays.