Tumor Growth in Experimental Animals Nutritional Manipulation and Chemotherapeutic Response in the Rat

Abstract

The effects of nutritional manipulation on host body weight dynamics, tumor growth patterns and host-tumor responses to chemotherapy were studied in Sprague-Dawley rats with Walker-256 carcinosarcomas. Group I maintained throughout on a regular diet (RD) gained carcass weight steadily. Group II lost carcass weight while fed a protein-free diet (PFD) but rapidly gained weight after switching to RD on day 15. Mean tumor volume increased 105% in group I from day 15 to 21, 218% in group II and 77% in group III (continued on PFD P < 0.05). From day 21 to day 33 tumor growth patterns were similar in groups I and II, while mean tumor volume eventually plateaued in group III. In study B, group II animals were given methotrexate (MTX; 20 mg/kg) 2 days and 6 days after switching from PFD to RD. The mean change in tumor volume in the MTX-treated rats was 1.31 .+-. 0.1 cm3 compared with 8.14 .+-. 0.1 cm3 (P < 0.001) in the saline-treated control rats. MTX did not significantly affect tumor growth patterns in group III (PFD) rats. In study A, protein-calorie malnutrition resulted in host carcass weight loss and tumor growth retardation while nutritional repletion restored host carcass weight and stimulated tumor growth. In study B, MTX was maximally effective in tumor-bearing rats that were switched from PFD to RD, demonstrating that nutritional manipulation can improve host nutritional status and increase tumor response to chemotherapy.